血电离和二次电喷射电离的比较 - - 高分辨率质谱仪用于实时呼吸分析
Jiafa Zeng1,2, Alexandra Christen3, Kapil Dev Singh4,2
1University Children's Hospital, CH-4056 Basel, Switzerland. jiafa.zeng@ukbb.ch.
Chimia
|December 9, 2023
概括
高分辨率质谱仪 (HRMS) 能够实时进行呼吸分析. 这项研究比较了二次电喷射电离 (SESI) 和血电离 (PI) 的呼吸分析,发现两者都是有效的,但有不同的信号噪声比.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 医学诊断 医学诊断 医学诊断
背景情况:
- 使用高分辨率质谱仪 (HRMS) 的实时呼吸分析提供了一种非侵入性的方法来获取生化信息.
- 这种技术对各种诊断和监测应用具有前景.
研究的目的:
- 直接比较两种电离技术的性能,二次电喷电离 (SESI) 和等离子电离 (PI),用于使用HRMS分析呼出的呼吸.
- 评估SESI-HRMS和PI-HRMS产生的质谱谱的可比性并确定其差异.
主要方法:
- 两个商业可用的电离源,SESI和PI,与相同的HRMS仪器相连.
- 从两个健康个体的呼吸样本进行了纵向分析,在两个平台上分析了58个标本.
- 数据分析的重点是检测到的特征数量和信号与噪声比 (S/N).
主要成果:
- 通过SESI-HRMS (2,209) 和PI-HRMS (2,296) 检测到大量的特征,其中60%的特征重叠.
- 信号与噪声比的显著差异被观察到:SESI-HRMS的S/N中位数为115 (IQR=408),而PI-HRMS的S/N中位数为5 (IQR=5).
- 各平台的质谱形状不同,这表明直接相互可比性存在挑战.
结论:
- 与HRMS相结合的SESI和PI都具有实时呼吸分析的能力,提供丰富的生物化学指纹.
- 观察到的S/N和光谱谱的差异突显出需要进一步研究,以优化每个技术,并了解其特定的代谢见解.
- 未来的工作应该集中在标准化方法和解释每个平台为临床应用生成的独特数据.
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