通过二次电喷射电离化质谱法进行直接呼吸分析的趋势用于临床应用
1ETH Zurich, Department of Chemistry and Applied Biosciences, Vladimir-Prelog-Weg 3, CH-8093 Zurich, Switzerland. streckenbach@org.chem.ethz.ch.
Chimia
|December 9, 2023
概括
二次电喷气电离高分辨率质谱 (SESI-HRMS) 分析出口呼吸以获得代谢洞察力. 这项技术推动了临床应用的生物标志物发现,尽管验证方面的挑战仍然存在.
科学领域:
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 呼出的呼吸含有内源和外源化合物,反映了代谢状态.
- 质谱仪,特别是SESI-HRMS,已经显著扩大了可检测的呼吸化合物.
- SESI-HRMS提供高灵敏度和多种化合物的同时检测.
研究的目的:
- 突出SESI-HRMS用于直接呼吸分析的能力.
- 强调SESI-HRMS在临床应用和生物标志物发现方面的潜力.
- 确定SESI-HRMS转化到临床环境中的当前挑战和未来方向.
主要方法:
- 使用二次电喷离子高分辨率质谱仪 (SESI-HRMS) 进行直接呼吸分析.
- 在临床试验中应用SESI-HRMS,用于在成人和儿童中发现生物标志物.
- 专注于用于临床转换的生物标志物识别和验证.
主要成果:
- 通过SESI-HRMS,可以对广泛的呼吸化合物进行敏感和同时检测.
- 在儿科和成人群体中成功展示了SESI-HRMS用于生物标志物发现的方法.
- 最近的研究重点是解决临床实施的挑战,包括验证.
结论:
- SESI-HRMS是一种强大的,非侵入性的工具,用于分析呼出的呼吸.
- 该技术在临床应用方面显示出显著的前景,特别是在生物标志物发现方面.
- 需要进一步的研究来克服生物标志物验证和临床翻译方面的挑战.
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