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来自BMSCs的外体通过携带miR-483-5p缓解高葡萄糖诱导的糖尿病视网膜病变

Dan Cao¹, Liang Zhou¹, Rong Hu²

¹Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, People's Republic of China.

Journal of biochemical and molecular toxicology

|December 9, 2023

概括

带有miR-483-5p的骨髓介质干细胞外体会抑制糖尿病视网膜病变 (DR) 的进展. 这一发现为DR提供了一个新的治疗策略,通过保护视网膜细胞免受高葡萄糖诱导的损伤.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

糖尿病视网膜病变 (DR) 是糖尿病的一个渐进的并发症.
骨髓介质干细胞 (BMSCs) 分泌的外体显示出调节DR的潜力.
在DR中的这些外体内,miR-483-5p的作用在很大程度上是未知的.

研究的目的:

调查携带miR-483-5p的BMSC衍生外体是否可以调节糖尿病视网膜病变 (DR) 的进展.
探索涉及胰岛素样生长因子1受体 (IGF-1R) 的潜在机制.

主要方法:

在实验室中,ARPE-19细胞被高葡萄糖 (HG) 处理,以模仿DR.
外体从BMSC中分离出来并进行了表征.
细胞活力,细胞亡,miR-483-5p和IGF-1R水平使用CCK-8,流式细胞计量,qRT-PCR和西白斑等测试进行了评估.
双化酶测定证实了miR-483-5p和IGF-1R之间的相互作用.

主要成果:

在ARPE-19细胞中,HG诱导了显著的亡.
由BMSCs衍生的外体保护ARPE-19细胞免受HG诱导的亡.
在外体体中抑制miR-483-5p加剧了细胞亡.
鉴定出IGF-1R是miR-483-5p的直接标,其沉默反转了对外体的保护作用,降低了miR-483-5p的调控.

结论:

由BMSCs衍生出来的外体通过输送miR-483-5p.p.来抑制DR的进展.
miR-483-5p/IGF-1R轴是这些外体的保护作用的关键机制.
这项研究为针对糖尿病视网膜病变的新型治疗策略提供了理论基础.

关键词:

这就是BMSCs.这就是为什么DR DR DR DR 在IGF-1RR中,IGF-1R是最常见的.外基因组是外基因组的组成部分.在 miR-483-5p 中使用.

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