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通过FACS从瘤微环境中分离活体免疫细胞

Aikaterini Kafka¹, Christos Ermogenous¹, Luigi Ombrato²

¹Centre for Tumour Microenvironment, Barts Cancer Institute, Queen Mary University London, John Vane Science Centre, London, UK.

Methods in molecular biology (Clifton, N.J.)

|December 9, 2023

概括

桃利基技术使得从转移性组织中分离出各种瘤微环境细胞,包括中性粒细胞,而无需视觉识别. 这种方法有助于早期癌症的研究和免疫细胞在转移性的特征.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

从瘤微环境 (TME) 中分离活细胞,特别是从转移性部位,是一项挑战.
在转移性组织中识别和分离特定细胞群需要先进的技术.

研究的目的:

介绍和演示Cherry-niche in vivo标签技术,用于从TME中分离细胞.
通过使用Cherry-niche来展示肺转移的中性粒细胞的隔离和表征.

主要方法:

利用Cherry-niche in vivo标记技术进行TME细胞隔离.
应用下游分析,包括Giemsa染色,反应性氧物种 (ROS) 检测,以及对隔离中性粒细胞的细胞分析.

主要成果:

通过使用Cherry-niche.成功地从肺转移的利基中分离出中性粒细胞.
证明了特征新鲜分离的中性粒细胞的可行性ex vivo.

结论:

桃是一种有效的方法,可以从转移性组织中分离各种TME细胞,包括中性粒细胞.
这项技术支持在转移性内的细胞的早期检测和表征,适用于其他免疫和非免疫细胞类型.

关键词:

细胞隔离细胞的隔离.流动细胞计量流动细胞计量免疫细胞是免疫细胞.标签标签标签标签标签转移转移转移转移中性粒细胞中性粒细胞.利基市场是一个利基.瘤微环境是一个微环境.

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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.