代谢压力环境驱动卵管上皮细胞的表观遗传修饰
Patricia Kubo Fontes1, Erika Cristina Dos Santos1, Heloise Cale da Rocha1
1Laboratory of Embryonic Metabolism and Epigenetic, Center of Natural and Human Science, Federal University of ABC, Santo André, São Paulo, Brazil.
Theriogenology
|December 9, 2023
概括
高血糖水平会影响卵巢外皮细胞 (OEC),改变诸如H3K9ac和线粒体功能之类的表观遗传标记. 培养基中的代谢物水平对于OEC研究至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 细胞的新陈代谢
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 卵管在伴生体成熟和早期胚胎发育中起着至关重要的作用.
- 卵巢细胞功能在雌性周期期间由类固醇激素动态调节.
- 代谢表观遗传学,即代谢和表观遗传修饰之间的相互作用,可能会增加卵管细胞调节的复杂性.
研究的目的:
- 为了研究牛卵管上皮细胞 (OEC) 中的代谢基因交叉.
- 为了确定改变葡萄糖水平对OEC代谢和表观遗传学 in vitro的影响.
- 评估雌性周期阶段对这些反应的影响.
主要方法:
- 初级OEC是在正常血糖和高血糖条件下体外培养的.
- 细胞从雌性周期的卵泡或状阶段的母牛中获取.
- 分析了线粒体膜潜能 (MMP),DNA甲基化 (5-甲基) 和基因素乙化 (H3K9ac).
- 用拉曼光谱法对培养介质代谢物进行了分析.
主要成果:
- 高血糖症在OEC中显著降低了H3K9ac水平和MMP.
- 血糖升高并没有改变5-甲基细胞素水平.
- 葡萄糖度是影响培养介质中的代谢物概况的主要因素,不论初始周期阶段如何.
- 在体外培养条件下观察到表观遗传重编程和能量代谢之间的关系.
结论:
- 代谢表观遗传调节影响卵管上皮细胞,特别是在改变的葡萄糖条件下.
- 试管培养系统虽然有用,但可能导致细胞脱差,影响结果.
- 培养媒介代谢物组成是涉及卵管细胞的研究中需要考虑的关键因素.
更多相关视频
09:12Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
Published on: November 14, 2018
8.0K
12:11Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Published on: May 11, 2017
11.0K
相关概念视频
02:07Oogenesis
63.7K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.7K
01:37Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
02:30Genomic Imprinting and Inheritance
34.5K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
34.5K