基于膜的高度高效和敏感的缩过程允许在大量废水中量化,监测和测序病毒
G El Soufi1, L Di Jorio2, Z Gerber3
1DHPI UR 7292, IUT Louis Pasteur, Université de Strasbourg, Schiltigheim, France; CNRS, IPHC, UMR 7178, Université de Strasbourg, Strasbourg F-67000, France.
Water research
|December 9, 2023
概括
新的废水方法提高了疫情监测的病毒检测灵敏度. 这些低成本的协议提高了SARS-CoV-2的量化和测序,即使在低度下也是如此.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 基于废水的流行病学为公共卫生监测提供了优势,但在敏感性和成本方面面临限制.
- 目前的方法难以检测低度的病毒,阻碍了疫情的早期检测和无症状病例的监测.
研究的目的:
- 开发和描述高度敏感,低成本和标准化的协议,用于从大量废水中集中和量化SARS-CoV-2.
- 评估这些方法对于病毒监测和测序的适用性.
主要方法:
- 一种常规的核酸提取技术 (5mL方法) 不依赖于商业套件或专用设备.
- 一种基于膜的新度 (MBC) 方法 (500毫升方法) 用于缩包裹 (SARS-CoV-2) 和非包裹病毒.
- 验证使用大量未经处理的废水的方法,并与现有技术进行比较.
主要成果:
- 与常规方法相比,MBC方法在量化SARS-CoV-2方面显著提高了灵敏度,检测的度低至每升40个基因组副本.
- 常规和MBC方法都与SARS-CoV-2测序兼容,测序质量与病毒基因组度相关.
- 即使在高度稀释的样本中,MBC方法也被证明是准确的,并且显示出在废水中检测特定酶抑制剂的潜力.
结论:
- 开发的MBC方法提供了一种高效,敏感和可重复的方法,用于从大量废水中定量和测序SARS-CoV-2.
- 这些低成本,可访问的协议可以加强基于废水的流行病学监测,特别是在资源有限的环境中.
- 这些发现有助于更可预测的病毒出现检测和标准化的实验室间比较.
相关概念视频
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
Methods to Assess Microbial Populations
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...


