到门还是不到门:通过流式细胞计指纹检测重新评估饮用水中的微生物
L Claveau1, N Hudson2, P Jeffrey1
1Cranfield University, College Road, Cranfield, Bedfordshire MK43 0AL, UK.
The Science of the total environment
|December 9, 2023
概括
动态封闭对于分析可变饮用水微生物质量至关重要,而静态封闭适合稳定的来源. 通过光指纹来补充细胞计数,可以提高水质评估.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 微生物学 微生物学
背景情况:
- 流细胞计 (FCM) 是微生物水质评估的一个关键方法.
- 门对于FCM至关重要,需要专业知识,并可能引入人为错误,阻碍方法标准化.
研究的目的:
- 分析微生物水质评估的多种流量细胞计门策略.
- 为了评估不同关方法对细胞计数准确性的影响.
- 为改善水行业微生物分析的最佳实践提供见解.
主要方法:
- 在流量细胞计散射图上对各种隔离方法的综合分析.
- 对不同门关策略对细胞计数影响的灵敏度分析结果.
- 使用了一年的饮用水样本数据集.
主要成果:
- 动态封锁对于分析高度可变的原始和分布式水是必不可少的.
- 静态门适用于稳定的水源和低细胞计数样品.
- 光指纹提供了更完整的了解微生物种群的变化.
结论:
- 细胞计数分析应补充光指纹,以进行全面的微生物评估.
- 建立每个水类型的动态基线对于选择适当的封闭策略至关重要.
- FCM指纹提供了一个动态的方法来量化处理过程,改善水的监测和控制.
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