功能性保存的PPARG外基环RNAs通过调节PPARG和HSL来增强肌肉内脂肪沉积
Ruirui Zhu1, Ye Feng1, Xintong Yang1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, Guangxi 530005, China.
International journal of biological macromolecules
|December 9, 2023
概括
过氧体增殖器激活受体马 (PPARG) 通过产生循环RNA (circRNAs) 来驱动肌肉内脂肪沉积. 这些circPPARG促进脂肪生成,并影响牲畜的肉质.
科学领域:
- 分子生物学分子生物学
- 动物科学动物科学
- 基因法规 基因法规
背景情况:
- 循环RNAs (circRNAs) 是生物过程中的关键调节者,如脂肪生成.
- 过氧体增殖器激活受体玛 (PPARG) 对于脂肪细胞的发育和肌内脂肪 (IMF) 沉积至关重要.
- 将PPARG与IMF存款联系在一起的监管机制尚未完全理解.
研究的目的:
- 为了研究PPARG衍生的circRNAs在调解肌肉内脂肪沉积中的作用.
- 阐明circPPARG在脂肪原分化和前脂肪细胞增殖中的作用.
- 探索circPPARGs的跨物种保护和体内影响.
主要方法:
- 来自PPARG的异构环RNAs的识别和表征.
- 在肌肉前脂肪细胞中进行功能性检测,以评估脂肪原体的分化和增殖.
- 研究circPPARG1与PPARG的相互作用及其对激素敏感脂酶 (HSL) 转录的影响.
- 在小鼠体内实验证实circPPARG1在IMF沉积中的作用.
主要成果:
- PPARG产生多个异构的circRNAs (circPPARGs),这些circRNAs中介于IMF的存储.
- 发现三个特定的circPPARG促进了基分化,并抑制了跨物种的前基细胞增殖.
- circPPARG1通过与PPARG相互作用并抑制HSL转录来抑制脂解.
- circPPARG1在小鼠体内对IMF存款产生了积极影响.
结论:
- PPARG通过其转录因子活动和circRNAs的产生来调节IMF存款.
- circPPARGs代表了脂肪生成和IMF沉积的新型监管途径.
- 这项研究增强了对PPARG在脂质代谢和肉质方面的多方面的作用的理解.
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