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长非编码RNAHOTAIR有助于关节特异性基因表达在类风湿性关节炎
Muriel Elhai1, Raphael Micheroli1, Miranda Houtman1
1Center of Experimental Rheumatology, Department of Rheumatology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland.
Nature communications
|December 9, 2023
概括
类风湿性关节炎 (RA) 关节炎显示了特定部位的基因表达差异. 膝关节中的长非编码RNAHOTAIR调节基因表达,并影响RA的进展.
科学领域:
- 分子生物学分子生物学
- 类风湿病学 类风湿病学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 类风湿性关节炎 (RA) 通常表现为对称关节卷入.
- 然而,不同的疾病模式表明RA进展中关节特异性的分子机制.
- 突纤维细胞 (SF) 在关节炎症中起着至关重要的作用.
研究的目的:
- 为了确定膝关节和手关节之间的RA突和SF的关节特异性分子差异.
- 调查长非编码RNAHOTAIR在RA的特定位点基因调节中的作用.
主要方法:
- 从膝关节和手关节进行RA突和SF的分析.
- 在SF的HOTAIR表达式的量化.
- 在HOTAIR敲除后,在SF中进行基因表达分析.
- 信号通路 (PI-Akt,IL-6,Wnt) 和细胞功能 (迁移,骨质细胞生成,B细胞招募) 的评估.
主要成果:
- 长非编码RNAHOTAIR仅在膝盖SF表达,并调节超过50%的特定位点基因表达.
- 霍泰尔的表达受到促炎性细胞因子的下调,并且在RA膝关节中相比于骨关节炎更低.
- 在膝盖SF中HOTAIR敲击改变PI-Akt,IL-6和Wnt信号,抑制SF迁移和骨质细胞生成,并增加B细胞的招募.
结论:
- 霍泰尔是一种关键的表观遗传调节器,用于关节特异性基因表达在类风湿性关节炎.
- 霍泰尔会影响关键的细胞过程参与RA的发病,包括炎症,骨质侵蚀和免疫细胞招募.
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