使用拉曼光谱和机器学习进行细胞培养特征的全面建模
Hiroki Tanemura1, Ryunosuke Kitamura2, Yasuko Yamada3
1Biologics Technology Research Laboratories I, Biologics Division, Daiichi Sankyo Co., Ltd., 2716-1, Aza Kurakake, Oaza Akaiwa, Chiyoda-Machi, Oura-Gun, Gunma, 370-0503, Japan. tanemura.hiroki.ij@daiichisankyo.co.jp.
Scientific reports
|December 9, 2023
概括
这项研究引入了一种自动机器学习方法,用于构建用于中国仓鼠卵巢 (CHO) 细胞培养监测的拉曼光谱模型. 这加快了许多代谢物的分析,改善了抗体药物生产.
科学领域:
- 生物技术是生物技术.
- 过程分析技术 (PAT) 是一种分析技术.
- 机器学习在生物处理中的应用
背景情况:
- 中国仓鼠卵巢 (CHO) 细胞对于生产基于抗体的治疗药物至关重要.
- 全面的代谢物监测对于优化CHO细胞培养和确保产品质量至关重要.
- 目前用于CHO细胞培养的拉曼光谱方法需要耗时的模型构建,阻碍了快速过程的发展.
研究的目的:
- 开发一种简单的,自动化的方法,用于构建用于CHO细胞培养的精确拉曼光谱模型.
- 通过机器学习和Python加速对众多代谢物的分析.
- 为了实现有效的过程优化和监控在制药制造业.
主要方法:
- 开发了一个使用Python和机器学习用于拉曼模型构建的自动化工作流.
- 采用贝叶斯优化来自动化和加速数据预处理和光谱范围选择.
- 利用各种机器学习算法 (线性回归,回归,XGBoost,神经网络) 来构建模型,将它们与部分最小平方 (PLS) 回归进行比较.
主要成果:
- 与传统的PLS回归相比,实现了更好的模型准确性.
- 展示了一种自动化方法,用于为100多个组件构建拉曼模型.
- 成功地促进了CHO细胞培养中的各种参数的持续监测.
结论:
- 自动机器学习方法显著提高了拉曼光谱模型开发的效率和准确性.
- 这种方法可以全面实时监测CHO细胞培养,支持更快的过程优化和强大的制造.
- 该研究为生物制药行业提供了一种有价值的工具,以改善抗体生产过程.
相关概念视频
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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