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史塔菲洛科克 (Staphylococcus epidermidis) 是一个更安全的替代金黄色葡萄球菌在测试面罩的细菌过效率的替代品

Silvia Chiera¹, Francesca Bosco², Chiara Mollea³

¹Department of Industrial Engineering, University of Trento, Trento, Italy.

Scientific reports

|December 9, 2023

概括

这项研究表明,使用 Staphylococcus epidermidis,一种更安全的细菌,可以有效地取代 Staphylococcus aureus 在面膜的细菌过效率 (BFE) 测试中. 这种方法保持了测试有效性,同时减少了实验室生物危害风险.

科学领域:

微生物学微生物学
材料科学材料科学材料科学
公共卫生公共卫生

背景情况:

面罩对于减轻空气传播病原体的传播至关重要.
细菌过效率 (BFE) 测试,使用金黄色葡萄球菌 (生物危害组2),是面具过性能的标准指标.
在实验室环境中处理金黄色葡萄球菌气溶会带来重大生物风险和可访问性挑战.

研究的目的:

在BFE测试中评估Staphylococcus epidermidis (生物危害组1) 作为金黄色葡萄球菌更安全的替代品.
为了确定S. epidermidis是否可以保持标准BFE测试协议的有效性和效率.
为了减少与实验室人员BFE测试相关的生物风险.

主要方法:

使用Staphylococcus epidermidis ATCC 12228.8.开发了一个修改后的BFE测试协议.
测试了三种代表性面罩模型 (外科和社区).
用S. epidermidis和S. aureus (标准方法) 的气溶对口罩进行了挑战.

主要成果:

使用S. epidermidis的细菌过效率 (BFE) 测试给出了与标准S. aureus方法相似的结果.
使用S. epidermidis保留了BFE测试的有效性和周转时间.
使用S. epidermidis显著降低了实验室操作人员的残留生物风险.

结论:

葡萄球菌表皮菌是一种可行的,更安全的替代品金黄色葡萄球菌对于面罩的BFE测试.
这种代用方法保持了过性能评估的完整性.
在BFE测试中实施S. epidermidis可以提高实验室的安全性,而不会损害科学严谨性.

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