低温大气等离子体对Shigella flexnerieri的杀菌作用
Yan Chen1, Yuanyuan He2,3, Tao Jin2
1Joint Laboratory of Plasma Application Technology, Institute of Advanced Technology, University of Science and Technology of China, Hefei, China.
Biomedical engineering online
|December 9, 2023
概括
寒冷的大气等离子体 (CAP) 有效地消毒Shigella flexneri,这是导致痢疾的一个原因. 由CAP产生的反应性氧物种 (ROS) 是负责细菌失活的主要代理物,提供了一个有前途的灭菌解决方案.
科学领域:
- 微生物学 微生物学
- 等离子体物理学的物理学
- 生物技术是生物技术.
背景情况:
- 西格拉柔性菌 (Shigella flexneri) 引起细菌性痢疾,特别是在资源较低的环境中.
- 开发快速,高效和环保的灭菌方法至关重要.
研究的目的:
- 为了研究冷大气等离子体 (CAP) 对S. flexneri.直接杀菌的疗效.
- 确定活性氧物种 (ROS) 在CAP诱导的细菌失活中的作用.
主要方法:
- 使用等离子体诊断来设置操作参数.
- 使用盘子计数和传输电子显微镜评估了细菌死亡率.
- 细胞内ROS和超氧化离子水平使用光探针进行测量.
主要成果:
- 在40秒内,CAP治疗实现了高达98.76%的细菌死亡率.
- 细胞内ROS水平随着血治疗持续时间的增加而增加.
- 鉴定出ROS是导致细菌死亡的主要因素.
结论:
- CAP提供了一种有效的灭菌方法,用于S. flexneri.
- 由CAP产生的ROS是细菌失活的主要驱动因素.
- CAP为医疗保健和日常生活提供了一个有前途的灭菌技术.
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