太空微重力增加了与人类心脏球体的增殖和分化相关的基因的表达
Hyun Hwang1, Antonio Rampoldi1, Parvin Forghani1
1Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA, USA.
NPJ microgravity
|December 9, 2023
概括
长期暴露在太空微重力中增强了人类诱导的多能干细胞衍生的心脏前体的分化和生长. 这表明微重力可能有利于心脏细胞发育,用于再生医学应用.
科学领域:
- 心血管生物学 心血管生物学
- 干细胞生物学 干细胞生物学
- 空间生物学 空间生物学
背景情况:
- 从人类诱导的多能干细胞 (hiPSCs) 有效生成心肌细胞对于研究和临床应用至关重要.
- 众所周知,空间微重力会改变细胞活动和功能.
- 以前的研究表明,短期暴露于微重力会在hiPSC衍生的祖先中调节心脏增殖基因.
研究的目的:
- 研究长期太空微重力暴露对hiPSC-心脏原始体的全球基因表达的影响.
- 评估长期微重力对心脏前体分化,增殖和功能的影响.
主要方法:
- 在国际空间站 (ISS) 中,在微重力和1g条件下,冷保存的3D hiPSC-心脏原始体在国际空间站 (ISS) 中培养了3周.
- 全球基因表达被使用RNA测序来分析.
- 进行了基因本体学和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 太空微重力调高了参与心脏分化,增殖和心脏结构/功能的基因.
- 与细胞外矩阵调节相关的基因在微重力下下调.
- 分析揭示了生物过程,分子功能和与细胞周期,心脏分化和功能相关的途径的丰富.
结论:
- 长期暴露在太空微重力中,对hiPSC-心脏原始体的分化和生长产生有益影响.
- 这些发现表明微重力在空间研究和再生医学中增强心脏细胞发育的潜在应用.
- 太空微重力可能成为优化心脏原生细胞疗法的宝贵工具.
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