在饮用水中溶解硫化不是动物研究中硫化的良好来源
Asghar Ghasemi1, Sajad Jeddi1, Nasibeh Yousefzadeh1
1Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Scientific reports
|December 9, 2023
概括
饮用水中的硫化物 (NaHS) 溶液不稳定,在12-24小时内失去大量的硫化 (H2S). 这种不稳定性使得NaHS在使用饮用水的动物研究中成为不可靠的H2S捐赠者.
科学领域:
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
- 动物生理学 动物生理学
背景情况:
- 硫化 (H2S) 在生理和病理方面起着至关重要的作用.
- 硫化 (NaHS) 是用于H2S研究的常见药理工具.
- 之前的研究已经在饮用水中使用了NaHS,假设溶液稳定性.
研究的目的:
- 为了确定30μM NaHS在12-24小时内饮用水中的稳定性.
- 评估NaHS在饮用水中的可靠性,作为动物研究中的H2S捐赠化合物.
主要方法:
- 在老鼠/小鼠水瓶中准备NaHS溶液.
- 硫化物度在不同的时间点 (0-24小时) 用甲蓝法测量.
- 血清硫化物水平在两周NaHS给药后被测量在老鼠中.
主要成果:
- 在12-24小时后,NaHS溶液显示出显著的不稳定性,从瓶尖损失72-75%.
- 在12-24小时后,瓶子内的NaHS度下降了47-72%.
- 在老鼠中,NaHS的使用并没有改变血清硫化物水平.
结论:
- 饮用水中的NaHS溶液是不稳定的,不能可靠地用于H2S捐赠.
- 这种给药途径导致对NaHS的暴露变化和减少.
- 研究人员应该重新考虑使用NaHS在饮用水中进行动物实验.
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