雌激素在多壁碳纳米管诱导的呼吸道炎症期间有助于M2a巨细胞的性别差异

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Jessica L Ray¹, Britten Postma¹, Rebekah L Kendall¹

¹Center for Environmental Health Sciences, University of Montana, Missoula, Montana, USA.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|December 10, 2023

概括

雌激素信号影响肺炎的性别差异,通过调节膜巨细胞 (AMs) 中的胆固醇和氧胆固醇水平. 这项研究阐明了雌激素如何影响M2a两极化和呼吸道炎症.

科学领域:

免疫学免疫学免疫学
内分泌学在内分泌学.
毒理学毒理学毒理学

背景情况:

2型炎症性肺部疾病在患病率和严重程度上显示出女性偏见.
多壁碳纳米管 (MWCNT) 暴露会诱导雌性中具有更明显的M2a巨表型的eosinophilic炎症.
雌激素受体 (ER) 信号与这些基于性别的巨细胞激活差异有关.

研究的目的:

研究雌激素受体信号在MWCNT诱导的肺炎中性别差异中的作用.
探索包括胆固醇和氧胆固醇调节在内的机制,这是雌激素对膜巨细胞 (AM) 现型的影响的基础.

主要方法:

在雄性和雌性小鼠中,在MWCNT灌注之前给予ER抗剂Fulvestrant.
分析了M2a基因表达,乙酸细胞数量和IL-33水平.
测量呼吸道氧化水平和胆固醇外流载体的表达 (Abca1, Abcg1).
在体外培养AMs与雌激素以评估细胞胆固醇和M2a两极化.

主要成果:

在雌性小鼠中,富勒弗兰特减弱了M2a基因表达和埃索诺菲利亚,同时在雄性小鼠中减少了IL-33和M2a基因.
与女性相比,暴露于MWCNT的男性的氧化醇水平 (25-OHC,7α,25-OHC) 较高.
在MWCNT暴露后观察到胆固醇外流载体Abca1和Abcg1的性别差异.
在体外雌激素暴露降低了胆固醇,提高了女性AMS的M2a反应,但降低了男性AMS的M2a偏振.

结论:

雌激素信号传递,可能通过调节 (氧) 和胆固醇流量,在调节AM表型方面发挥着重要作用.
这些机制有助于在暴露于MWCNT后的呼吸道炎症中观察到的性别差异.
向雌激素介导途径可能为性别偏差肺部疾病提供治疗策略.

关键词:

一个全额补助金.碳纳米管是如何使用的胆固醇胆固醇胆固醇是一种雌激素雌激素雌激素是一种这是一种炎症炎症炎症炎症.肺肺肺肺肺肺肺肺肺巨细胞是一个巨细胞.氧化胆固醇是一种氧化醇.呼吸道呼吸道呼吸道在性爱中,性爱是性爱.

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