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单一植物中双特异单克隆抗体的产生,表达和功能

Caiquan Jin¹, Yang Joo Kang¹, Se Ra Park^1,2

¹Department of Medicine, College of Medicine, Chung-Ang University, Seoul, 06974, South Korea.

|December 10, 2023

概括

这项研究表明,大型单链 (LSC) CO17-1AK和抗人类表皮生长因子受体2 (HER2) VHH-FcK抗体可以在单一的转基因植物中产生. 这些植物产生的抗体表现出类似的抗瘤活性,并向特定的癌症细胞系.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
植物科学植物科学

背景情况:

重组单克隆抗体 (mAbs) 对于癌症治疗至关重要,但通常需要复杂的哺乳动物,昆虫或细菌系统.
植物表达系统提供了诸如成本效益,可扩展性和缺乏人类病原体的抗体生产等优势.

研究的目的:

为了产生一种单一的转基因植物,共同表达抗结直肠癌LCS CO17-1AK和抗HER2 VHH-FcK抗体.
评估这些共同表达的植物性抗体的产生,向结合和抗瘤活性.

主要方法:

单独设计的植物交叉授粉,以表达LCS CO17-1AK和抗HER2 VHH-FcK.
使用PCR和西斑分析对F1后代的查.
通过细胞ELISA对抗体结合的评估和使用SW620和SKBR-3癌细胞系的伤口愈合试验的功能性抗瘤活性.

主要成果:

成功生成了转基因F1兄弟姐妹,共同表达了LSC CO17-1AK和抗HER2 VHH-FcK抗体.
证实了两种抗体在结直肠 (SW620) 和乳腺 (SKBR-3) 癌细胞上的各自标蛋白的特定结合.
由各自的抗体显著抑制SW620和SKBR-3细胞迁移,与父母抗体相当.

结论:

一个单一的植物可以被设计为共同生产功能性的LSC CO17-1AK和抗HER2 VHH-FcK抗体.
植物性抗体对目标癌细胞具有与传统抗体相似的结合和抗迁移作用.
这项研究验证了基于植物的表达系统作为生产用于癌症治疗的治疗抗体的可行平台.

关键词:

癌症癌症癌症癌症交叉授粉是一种交叉授粉.在LSC的抗体.来自植物的抗体.再组合蛋白质是一种重组蛋白质.转基因植物是转基因植物.在VHH抗体中,VHH的抗体是:

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