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间歇性禁食会破坏海马体依赖的记忆和老年雄性和雌性小鼠的北上腺素含量

Kimberly Wiersielis¹, Ali Yasrebi², Thomas J Degroat³

¹Department of Animal Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, United States; Joint Graduate Program in Toxicology, Rutgers, The State University of New Jersey, Piscataway, NJ, United States.

Physiology & behavior

|December 10, 2023

概括

间歇性禁食 (IMF) 根据年龄对雄性和雌性小鼠的情绪和认知有不同的影响. 这项研究研究了IMF对类似焦虑的行为,记忆和大脑基因表达的影响,揭示了性别和年龄相关的变化.

科学领域:

神经科学是一个神经科学.
内分泌学在内分泌学.
行为科学行为科学

背景情况:

间歇性禁食 (IMF) 与健康益处有关,但其对情绪和认知的影响尚不清楚.
以前的研究表明,IMF可能会影响下丘脑中的神经递质系统和基因表达,可能会影响认知功能.
关于IMF与焦虑的关联存在有限的数据,尽管一些人体研究表明潜在的益处.

研究的目的:

研究IMF对中年和老年雄性和雌性小鼠焦虑类行为,记忆和神经化学变化的影响.
探索IMF如何影响与情绪和认知相关的关键大脑区域的基因表达,例如弧形核,侧面下丘脑和心脏部位.
确定IMF对认知处理和神经生物学标志物的性别和年龄特定影响.

主要方法:

不同年龄 (12和18个月) 和性别的小鼠接受了间歇性禁食 (IMF) 饮食.
用开放场测试来评估类似焦虑的行为.
认知功能,包括海马体依赖和独立记忆,使用Y迷宫,空间物体识别和新型物体识别测试进行评估.
在背部海马体 (DH) 和前额叶皮层 (PFC) 通过ELISA测量了上腺素 (NE) 含量.
基因表达分析在弧形细胞核 (ARC),侧向下丘脑 (LH) 和基因表达位 (LC) 中进行.

主要成果:

国际货币基金组织改善了女性的空间导航,但损害了空间对象定向.
新型物体识别记忆在12个月大的男性中得到改善,但在12个月大的女性中下降.
18个月大的雌性表现出减少了类似焦虑的行为和增加了运动.
在DH中观察到与性别和年龄相关的北上腺素水平变化.
在ARC,LH和LC中,IMF改变了基因表达 (Agrp,Npy,Adr1a,Cart,Npy5r,Adr1b,Npy1r,Hcrt),具有显著的性别差异.

结论:

间歇性禁食诱导了小鼠的心情,认知和神经化学特征的显著变化.
国际货币基金组织的影响高度依赖于动物的性别和年龄.
国际货币基金组织对调节情绪和认知功能的关键大脑区域的神经递质系统和基因表达产生影响,突显了其影响的复杂性.

关键词:

焦虑焦虑是一种焦虑.认知认知是一种认知.在海马体内,海马体断断续续的禁食是一种禁食.上腺素 (Norepinephrine) 是一种可以前额叶皮层前额叶皮层.

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