功能冗余的甲酸盐脱酶使甲球菌 (Methanococcus maripaludis) 的成长能够依赖甲酸盐
Mohd Farid Abdul Halim1, Dallas R Fonseca1, Thomas D Niehaus1
1Department of Plant and Microbial Biology, University of Minnesota, Twin Cities, St. Paul, Minnesota, USA.
The Journal of biological chemistry
|December 10, 2023
概括
甲基生物利用甲酸盐脱酶 (Fdh) 来获得能量. 在Methanococcus maripaludis中,Fdh的两种异型都是功能冗余的,其活性可能受到基因调节或金属可用性所限制.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 甲基生物对于无氧环境中的有机物质再矿化至关重要.
- 性甲基生成使用H2来减少CO2到CH4;酸盐可以替代H2.
- 甲酸脱酶 (Fdh) 对于甲酸氧化至关重要,将其与辅酶F420减少或异硫化酶 (Hdr) 催化反应联系起来.
研究的目的:
- 在生物化学上描述两种Fdh异型,它们与*Methanococcus maripaludis*的甲基生成有关.
- 阐明Fdh异构体在甲生成途径中的作用和相互作用.
主要方法:
- Fdh异型体的生物化学表征.
- 对与Hdr.的酶相互作用的分析.
- 评估F420的减少和基于黄素的电子分支.
- 对突变菌株 (Δfdh1) 和抑制基因突变 (moeA) 的遗传分析.
- 对Fdh异型的金属含量分析.
主要成果:
- Fdh1和Fdh2都与Hdr相互作用,用于基于黄素的电子分支,并以相似的速度降低F420.
- 对于这两种酶来说,F420的减少先于基于黄素的电子分支.
- 在Δfdh1突变体中,Fdh2活性需要抑制器突变 (moeA损失),从而实现生长.
- 异形活性似乎取决于或的可用性.
结论:
- 在 *M. maripaludis* 中,两个 Fdh 异型是功能冗余的.
- 在Fdh异构体的体内活性可能由基因表达或金属辅因子的可用性来调节.
- 这项研究增强了对甲酸盐氧化和甲基生成中的Fdh功能的理解.
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