Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 8, 2025

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry
12:31

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Published on: December 8, 2015

15.1K

没有陶氏病的陶氏播种.

Michael S LaCroix1, Efrosini Artikis2, Brian D Hitt3

  • 1Center for Alzheimer's and Neurodegenerative Diseases, Peter O'Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

The Journal of biological chemistry
|December 10, 2023
PubMed
概括

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Multisite study: Predicting Lewy body disease using skin biopsy α-synuclein seed amplification assays.

Journal of neuropathology and experimental neurology·2026
Same author

Brain-Only Versus GI-Only Synucleinopathy: A Comprehensive Autopsy Study With Both IHC and SAA.

medRxiv : the preprint server for health sciences·2026
Same author

Amyloid fibril polymorphism in the heart and liver of a patient with polyneuropathic ATTRv-V122Δ amyloidosis.

Communications biology·2026
Same author

'Tripod-like' lung-targeting (LuT) lipids for highly efficient and selective LNPs for gene delivery and editing.

Nature biomedical engineering·2026
Same author

Differential patterns of central synucleinopathy and catecholaminergic abnormalities in Lewy body diseases and multiple system atrophy.

Parkinsonism & related disorders·2026
Same author

Correction to "Design of Tau Aggregation Inhibitors Using Iterative Machine Learning and a Polymorph-Specific Brain-Seeded Fibril Amplification Assay".

Journal of the American Chemical Society·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
查看所有相关文章
此摘要是机器生成的。

健康的大脑含有tau播种活动,表明正常功能可能在阿尔茨海默病 (AD) 中变得致病. 这种类似子的传播在构造上不同于AD tau.

科学领域:

  • 神经科学是一个神经科学.
  • 生物化学 生物化学

背景情况:

  • 包括阿尔茨海默氏症 (AD) 在内的神经退行性病症的特征是大脑中蛋白聚集的积累.
  • 新出现的证据表明,可能充当子,能够自我传播和细胞和生物之间传播.
  • 这种类似子的行为表明复制的细胞机制,可能与种子的正常生理作用有关.

研究的目的:

  • 在健康的人类大脑组织中调查tau播种活动的存在.
  • 描述在非病理性大脑中发现的种子的特性.
  • 为了比较健康对照组的种子与阿尔茨海默病中发现的种子.

主要方法:

  • 开发和利用一种针对种子的新型单克隆抗体 (MD3.1).
  • 从健康个体 (19-65岁) 的头顶和小脑皮层中的免疫净化.
  • 使用直角试验检测播种活动,包括实时震动诱导转换 (RT-QuIC) 和抗体结合配置文件.

主要成果:

  • 在健康对照者体皮层中检测到tau播种活动,但在小脑中没有.
  • 从野生型或人类tau knockin小鼠的大脑同质体中没有观察到播种活动.
  • 播种活动与受试者的年龄或整体大脑tau水平无关.
  • 形状分析显示,对照种子与AD种子不同,这表明结构组合不同.
关键词:
阿尔茨海默氏症是阿尔茨海默氏症的一种疾病.在FRET生物传感器.健康的大脑健康的大脑普里昂是什么意思?普里昂是什么意思?陶播种活动播种活动病症是一种病症.

更多相关视频

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
09:49

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

18.7K
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

18.4K

相关实验视频

Last Updated: Jul 8, 2025

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry
12:31

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Published on: December 8, 2015

15.1K
In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
09:49

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

18.7K
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

18.4K

结论:

  • 健康的大脑具有内源性种活动,支持种子正常生理功能的假设.
  • 细胞和遗传背景似乎对培育具有种子能力的的发展至关重要.
  • 对照对抗AD的种子的独特形状特征表明,在疾病状态下,正常功能可能会变得异常.