泌尿病原性大肠杆菌 (Escherichia coli) 在 siderophores 形式使用肠杆菌素衍生的催化剂
Zongsen Zou1, John I Robinson1, Lindsey K Steinberg1
1Center for Women's Infectious Diseases Research, Washington University School of Medicine, St Louis, Missouri, USA; Division of Infectious Diseases, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA.
The Journal of biological chemistry
|December 10, 2023
概括
泌尿病原性大肠杆菌 (UPEC) 使用肠杆菌的 siderophores 铁. 这些分子的不完全分解允许UPEC获得更多的铁,最大限度地提高生物合成的营养回报.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 泌尿病原性大肠杆菌 (UPEC) 需要铁来治疗尿路感染.
- 在UPEC中,合成像菌素 (Ent) 这样的 siderophores,以获得铁.
- siderophore 生物合成的代谢成本表明有显著的益处.
研究的目的:
- 调查肠杆菌素及其分解产品在UPEC铁获取中的作用.
- 确定外代谢物是否是中间体或代谢产物.
- 了解UPEC是如何最大限度地从 siderophores 获得铁的.
主要方法:
- 利用定义的肠杆菌素生物合成和进口突变物.
- 在对铁有反应的条件下分析了细胞外代谢组.
- 具有特征的低分子量二度外代谢物.
主要成果:
- 肠杆菌素和相关产品是UPEC细胞外代谢中的关键.
- 外代谢产物是不完整的 siderophore 代谢的产物.
- 不完全化的肠杆菌素衍生物保留了铁清除活性.
结论:
- UPEC不完全化肠杆菌素以最大限度地增加铁的吸收.
- katabolic 的低效率使得每个 siderophore 可以获得多个铁离子.
- 这一策略提高了 siderophore 生物合成投资的营养回报.
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