异常的卵泡调节T细胞与异常的卵泡调节T细胞与异常的卵巢淋巴细胞组织的鼻息肉中的免疫球蛋白过度生产有关
Jia Song1, Hai Wang1, Zhe-Zheng Wang1
1Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Hubei Clinical Research Center for Nasal Inflammatory Diseases, Wuhan, China.
The Journal of allergy and clinical immunology
|December 10, 2023
概括
鼻息肉中的毛囊调节T细胞 (TFR) 由于CTLA-4和维生素D受体表达的降低,其抑制免疫球蛋白生产的功能减弱. 在体外维生素D治疗恢复了TFR细胞抑制能力.
科学领域:
- 免疫学 免疫学 免疫学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 细胞生物学 细胞生物学
背景情况:
- 鼻息肉中的异位淋巴组织 (eLTs) 具有毛囊辅助T (TFH) 细胞,驱动局部免疫球蛋白过度生产.
- 毛囊调节性T (TFR) 细胞通常会抑制二级淋巴体器官的免疫球蛋白产生,但它们在NP eLTs中的作用尚不清楚.
研究的目的:
- 调查鼻 (NP) 中TFR细胞的存在,表型和功能.
主要方法:
- 利用单细胞RNA测序,免疫光染色和流细胞计来分析NP中的TFR细胞.
- 与自主B细胞共同培养排序的多和循环T细胞子集,以通过ELISA评估免疫球蛋白的产生.
主要成果:
- 在NP eLTs中发现了TFR细胞,与对照组相比,NP中的TFR/TFH比率和TFR/TFH比率下降.
- 多重体TFR细胞表现出CTLA-4和维生素D受体表达的减少,损害了它们抑制TFH细胞诱导免疫球蛋白产生的能力.
- 在体外维生素D治疗增强了多重体TFR细胞上的CTLA-4表达,并恢复了它们的抑制功能.
结论:
- eLT中的鼻息肉TFR细胞显示CTLA-4和维生素D受体表达减少,导致TFH细胞活性受损.
- 在实验室中,维生素D治疗表明有可能逆转TFR细胞的抑制功能障碍.
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