在RSV纳入体内,PABP驱动的二次凝缩阶段激活病毒mRNA用于核糖体招募
Qiang Zhang1, Hanzhe Ye1, Cong Liu1
1State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Virologica Sinica
|December 10, 2023
概括
呼吸道同胞性病毒 (RSV) 包容体包含由二次液-液相分离形成的无M2-1病毒mRNP凝聚物. 这个过程集中了病毒mRNA和翻译因子,激活它们用于核糖体招募.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 呼吸道同胞性病毒 (RSV) 包容体 (IBs) 通过液体-液体相分离 (LLPS) 形成.
- 内部结构,IB相关颗粒 (IBAGs),集中病毒M2-1抗终结因子和mRNA,但它们的形成和功能尚不清楚.
研究的目的:
- 阐明RSV IBs中IBAG形成的机制.
- 确定IBAG在病毒复制中的生理功能.
主要方法:
- 研究了RSV IBs内部结构的组成和形成.
- 利用生物化学分析来研究蛋白质-蛋白质和蛋白质-RNA相互作用.
- 分析了PABP和翻译启动因子在mRNP凝结物形成中的作用.
主要成果:
- RSV IBs含有由二次LLPS形成的M2-1自由病毒信使核糖蛋白 (mRNP) 凝聚物.
- RSV核蛋白 (N) 和M2-1将PABP招募到IB中,促进PABP与病毒mRNA结合和二级相分离.
- PABP-eIF4G1相互作用调节了mRNP凝聚物组成,招募翻译因子 (eIF4G1,eIF4E,eIF4A,eIF4B,eIF4H) 来激活病毒mRNA进行翻译.
结论:
- 在RSV感染期间提出了一种新的LLPS调节的翻译机制.
- 确定了二次凝结相作为新型抗病毒策略的潜在目标.
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