从临床环境中分离出来的产生大肠杆菌的CTX-M型ESBL的流行病学和分子特征
Keyi Yu1, Zhenzhou Huang2, Yue Xiao1
1National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), State Key Laboratory of Infectious Disease Prevention and Control, Beijing, 102206, China; Center for Human Pathogenic Culture Collection, China CDC, Beijing, 102206, China.
Journal of global antimicrobial resistance
|December 10, 2023
概括
这项研究揭示了大肠杆菌中的各种blaCTX-Ms基因型,将序列类型与临床起源联系起来. 了解这些联系有助于预测和控制抗菌素耐药性的传播.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学是一种遗传学.
- 流行病学 流行病学
背景情况:
- 在全球范围内,blaCTX-Ms在大肠杆菌中越来越主导扩展谱β-乳糖酶 (ESBLs).
- 了解blaCTX-Ms的遗传多样性和传播对于打击抗菌素耐药性至关重要.
研究的目的:
- 系统地研究大肠杆菌序列类型 (ST),临床起源和blaCTX-Ms基因型之间的关系.
- 分析不同blaCTX-Ms变异在临床大肠杆菌分离物中的流行率和分布.
主要方法:
- 来自NCBI的1005个完整的临床大肠杆菌序列的分析.
- 多位数序列类型 (MLST) 用于确定序列类型 (ST).
- 查抗生素耐药性基因,包括blaCTX-Ms,卡巴胺耐药性基因和mcr基因.
主要成果:
- 大肠杆菌分离物主要来自便,尿液和血液,属于153个STs.
- 观察到34.7%的blaCTX-Ms阳性率,其中 blaCTX-M-55-like, blaCTX-M-1-like和 blaCTX-M-2-like是主要的基因型.
- 在特定的STs (例如ST2,ST43,ST8) 和特定的blaCTX-Ms基因型之间发现了显著的关联,这表明了传播模式.
结论:
- 大肠杆菌表现出越来越多的blaCTX-Ms基因型的多样性.
- 大肠杆菌STs和blaCTX-Ms基因型之间的已识别的联系可以为预测和减轻耐药性传播的策略提供信息.
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