肝脏缺血-再输液损伤转录组的患者间异质性:对研究和诊断的影响
Silvia Groiss1, Christian Viertler1, Marcel Kap2
1Diagnostic & Research Institute of Pathology, Medical University of Graz, 8010 Graz, Austria.
New biotechnology
|December 10, 2023
概括
手术压力和缺血-再输血损伤显著改变肝脏基因表达,使分子诊断复杂化. 研究人员确定了关键基因通路和230个强大的参考基因,以提高诊断准确度.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 分子生物学分子生物学
- 手术病理学手术病理学
背景情况:
- 外科手术和缺血-再输液损伤 (IRI) 可以诱导细胞反应,显著改变转录组概况.
- 这些分析前的变化使得肝脏组织中精确的分子诊断变得复杂.
研究的目的:
- 描述手术患者的非恶性肝脏样本中对转录组概况的分析前效应.
- 为了识别强大的基因签名用于诊断应用,尽管手术和IRI诱导的改变.
主要方法:
- 在30名患者的143个肝脏样本中,使用Affymetrix微阵列对转录形状的分析.
- 通过逆转录聚合酶链反应 (RT-PCR) 验证信使RNA (mRNA) 表达.
- 基因表达在不同手术技术 (普林格尔手术与完全血管排除) 和时间点之间的比较.
主要成果:
- 确定了179个放松调节的基因,突出显示了在缺血期间细胞因子信号传递和NFκB通路激活的增加.
- 重灌诱导了涉及TNF,NFκB和MAPK通路的亲细胞断裂和亲炎症级联.
- 在非瘤组织中发现了癌症的分子特征,并确定了230个分析前强大的基因,可作为基因参考.
结论:
- 手术和IRI等预分析因素对肝脏转录组产生深远的影响,需要在分子诊断中仔细考虑.
- 确定了一组230个强大的基因,为肝病的未来诊断应用提供了潜在的稳定参考基因.
- 基因表达反应的患者间变异性表明压力反应和疾病影响的个体差异.
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