赫利科维尔帕阿米杰拉 (Helicoverpa armigera) 的ATP结合磁带输送器ABCA2是一种Bacillus thuringiensisCry2Ab毒素的功能性受体
Chunxia Gan1, Zheng Zhang1, Zeng Jin1
1College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Pesticide biochemistry and physiology
|December 10, 2023
概括
甲状腺菌 (Bt) 在Helicoverpa armigera中对Cry2Ab毒素的耐药性与ABCA2基因有关. 这项研究揭示了HaABCA2作为Cry2Ab受体,对昆虫细胞中毒和毛孔形成至关重要.
科学领域:
- * 昆虫学 昆虫学
- * 分子生物学 * 分子生物学
- * 生物化学 * 生物化学
背景情况:
- *Bacillus thuringiensis (Bt) 晶体 (Cry) 蛋白质对于转基因作物的害虫控制至关重要.
- *Cry2Ab毒素在全球范围内用于对抗类虫虫害,如Helicoverpa armigera.
- *对Cry2Ab的耐药性与ABCA2基因的突变有关,但其确切作用尚不清楚.
研究的目的:
- * 为了阐明Helicoverpa armigera ABCA2 (HaABCA2) 蛋白在Cry2Ab中毒中的功能作用.
- * 确定HaABCA2的区域对于Cry2Ab结合和毒性至关重要.
主要方法:
- *使用Sf9昆虫细胞和Xenopus laevis卵细胞的体外表达系统.
- * 在表达野生型和突变 HaABCA2.2 的细胞中评估了 Cry2Ab 结合和敏感性.
- * 在Cry2Ab暴露时表达HaABCA2的卵细胞中测量离子膜流量.
主要成果:
- *Cry2Ab与表达HaABCA2的Sf9细胞特别结合,从而产生剂量依赖的敏感性.
- * 缺少关键细胞外或细胞内循环区域的突变HaABCA2失去了对Cry2Ab的敏感性.
- *在Cry2Ab的存在下,HaABCA2促进了离子膜流动,这表明它在孔隙形成中的作用.
结论:
- * HaABCA2 作为 Helicoverpa armigera 中 Cry2Ab 毒素的关键受体起作用.
- * HaABCA2 的特定细胞外和细胞内区域对于 Cry2Ab 相互作用和中毒是必不可少的.
- *HaABCA2在促进Cry2Ab孔隙形成中发挥着关键作用,导致杀虫活性.
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