在乳腺癌中构建和分析伪基相关的ceRNA网络
Hossein Mohebifar1, Amir Sabbaghian1, Touraj Farazmandfar1
1Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Shastkola Road, Falsafi Complex, Gorgān, 4934174611, Iran.
Scientific reports
|December 10, 2023
概括
这项研究表明,涉及伪基因的GBP1/hsa-miR-30d-5p/GBP1P1轴在乳腺癌 (BC) 中起着致瘤作用. 过度表达伪基因GBP1P1增强了BC细胞的致癌性质,为BC发育提供了新的见解.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 乳腺癌 (BC) 仍然是女性癌症死亡的主要原因.
- 伪基因曾经被认为是非功能性的,现在越来越多地被认为是它们在疾病中的调节作用.
研究的目的:
- 调查伪基因在乳腺癌发病过程中的潜在作用.
- 构建和分析竞争的内源RNA (ceRNA) 网络,以确定关键的调节轴.
主要方法:
- 在九个BC数据集中对差异表达基因的查.
- 使用基因-伪基因对和相互作用的微RNA (miRNAs) 构建一个ceRNA网络.
- 在患者样本和细胞系中确定GBP1/hsa-miR-30d-5p/GBP1P1轴的验证.
主要成果:
- 确定了一个关键的ceRNA模块,突出了GBP1/hsa-miR-30d-5p/GBP1P1轴.
- 在BC组织中,GBP1及其假基因GBP1P1被上调,而hsa-miR-30d-5p被下调.
- 在MCF-7细胞中,GBP1P1的过度表达增加了GBP1,降低了hsa-miR-30d-5p,并增强了细胞活力,克隆原性和迁移.
结论:
- GBP1/hsa-miR-30d-5p/GBP1P1轴作为乳腺癌的潜在致癌驱动因素.
- 伪基因,特别是GBP1P1,可以通过ceRNA网络影响BC进展.
- 这项研究为伪基因在BC发育中的作用提供了新的见解,并提供了潜在的治疗点.
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