埃梅里亚形菌的细胞外囊泡在宿主细胞中差异地表达 lncRNAs
Joshua S Olajide1,2, Zigang Qu1, Shunli Yang1,3
1State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
The Journal of eukaryotic microbiology
|December 11, 2023
概括
埃梅里亚形虫杂虫的细胞外囊泡 (EVs) 改变宿主肠道上皮细胞 (IEC) 的RNA表达,包括长非编码RNA (lncRNAs). 这些差异表达的lncRNAs可以作为Eimeria感染的诊断标记.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 长非编码RNAs (lncRNAs) 在原生动物感染期间调节宿主肠道上皮细胞 (IECs).
- 猿类复合体Eimeria falciformis sporozoite细胞外囊泡 (EVs) 携带毒性因子,影响宿主免疫反应和促炎性基因表达.
研究的目的:
- 为了研究Eimeria falciformis EVs在宿主IEC上的生物活性.
- 为了识别宿主RNA表达变化,特别是lncRNAs,由E. falciformis引发的EVs.
- 探索差异表达的lncRNAs作为Eimeria感染的诊断标记物的潜力.
主要方法:
- E. falciformis sporozoites与非活化的宿主细胞的相互作用.
- 通过超离心和密度梯度介质隔离和净化寄生虫EVs.
- RNA测序,功能注释和定量PCR用于分析EVs对宿主细胞RNA的剂量依赖作用.
主要成果:
- 在小鼠IEC中,E. falciformis EVs诱导了mRNA,circRNA和lncRNA的表达.
- 观察到309个lncRNA (157个上调,152个下调) 的显著差异表达.
- 差异表达的lncRNA与免疫信号通路 (FoxO,NF-κB,MAPK,TGF-β),细胞因子和热相关.
结论:
- 在IEC中,E. falciformis EVs显著改变宿主细胞RNA表达特征.
- 差异表达的lncRNAs代表了对Eimeria感染的潜在诊断生物标志物.
- 主体RNA的EV介导调制突出了寄生虫与主体相互作用的新机制.
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