解精子中的Na/K-ATPase与格斯公牛的生育能力之间的关系
Juliana Carla Cavalcanti Marques1, Allan Rodolf Ribeiro Cezar1, Agnelo Douglas do Nascimento1
1Laboratório de Reprodução Animal, Universidade Federal de Alagoas, Viçosa, AL, Brasil.
Animal reproduction
|December 11, 2023
概括
安格斯公牛精子中的-ATPase (Na/K-ATPase) 活性和表达与生育不相关. 然而,精子动力学可能表明高生育力公牛的冷耐受性更高.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物化学 生化学
背景情况:
- 预测公牛的生育率对于畜牧业的经济成功至关重要.
- 在公牛中识别可靠的生育标记仍然是一个重大挑战.
研究的目的:
- 为了研究- ATPase (Na/K-ATPase) 在高生育率 (HF) 和低生育率 (LF) 安格斯公牛的解精子中的活性和表达.
- 评估 Na/K-ATPase 和解后和化期间的精子质量参数之间的关系.
主要方法:
- 从HF和LF安格斯公牛 (每只n=4) 收集精子样本.
- 在解后评估Na/K-ATPase活动.
- 评估精子动力学,膜完整性和Na / K-ATPase表面表达,在解后立即进行,并在化120分钟后进行.
主要成果:
- 在任何化时间,HF和LF公牛之间的精子膜完整性,动力学或Na/K-ATPase表达没有显著差异.
- 与LF公牛相比,在HF公牛中观察到更高Na/K-ATPase酶活性的趋势 (P=0.06).
- 与过度激活相关的精子动力学显示,在高压公牛中,可能表明更高的低温耐受性.
结论:
- 在解的安格斯公牛精子中,Na/K-ATPase活性和表达与固定时间人工授精后的生育指数没有直接联系.
- 精子动力学,特别是那些与过度激活相关的,可以作为高生育性公牛精子高冷耐性的指标.
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