PEDOT:用于神经刺激的PSS涂层金电极
Gerwin Dijk, Jolien Pas1, Katarina Markovic2
1Panaxium SAS, Aix-en-Provence 13100, France.
APL bioengineering
|December 11, 2023
概括
用PEDOT:PSS涂抹金电极可以提高神经刺激的安全性和寿命. 这种导电聚合物保护电极免受腐蚀,并在长时间的电刺激过程中显著改善神经元细胞存活率.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 神经科学是一个神经科学.
背景情况:
- 安全和长期的神经刺激需要电极,可以注入电荷,而不会损伤组织或降解.
- 像PEDOT:PSS这样的导电聚合物具有高电容,这使得它们对电极修改具有前景.
- PEDOT:用于神经刺激的PSS涂层电极的稳定性和安全性尚不清楚.
研究的目的:
- 为了评估PEDOT:PSS涂层 (Pt-PEDOT:PSS) 微电极用于神经刺激的性能.
- 为了比较Pt-PEDOT:PSS电极与裸 (Pt) 电极的稳定性,安全性和生物相容性.
- 研究PEDOT:PSS涂层对电极完整性和神经元细胞存活的保护作用.
主要方法:
- Pt-PEDOT的制造和特征:PSS和赤裸的Pt微电极.
- 在不同电荷密度 (64255 μC cm−2) 的电流控制的两相电刺激脉冲的传递.
- 通过腐蚀分析评估电极降解,并使用作为神经圈培养的原发皮质细胞评估神经元细胞存活率.
主要成果:
- 裸体Pt电极在刺激2小时后出现了显著的腐蚀,而Pt-PEDOT:PSS电极保持完整.
- 两种电极类型都表现出类似的充电密度值,用于急性刺激 (127μC cm-2),在较高密度下增加反应.
- 与裸体Pt电极相比,Pt-PEDOT:PSS电极的神经元细胞存活率显著更高,特别是在长时间 (2小时和6小时) 的刺激后.
结论:
- PEDOT:PSS涂层可以有效地防止在电刺激过程中金电极腐蚀.
- Pt-PEDOT:PSS微电极提供保护作用,增强神经元细胞存活率并减少组织损伤.
- PEDOT:PSS是开发更安全,更耐用的电极的有希望的材料,用于长期的神经刺激应用.
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