深性溶剂对细胞酶和乳酸酶的活性和稳定性的影响
Jianjun Guo1,2, Nanshan Zhao1, Yaxin Zhao1
1School of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310000, China.
ACS omega
|December 11, 2023
概括
深度浸泡溶剂 (DES) 为酶储存提供了一个可持续的替代方案. 这项研究表明,DES的最佳含水量增强了酶的稳定性,为长期的酶保存提供了基础.
科学领域:
- 绿色化学 绿色化学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 深度浸泡溶剂 (DES) 是对离子液体的经济高效,环保的替代品.
- 酶稳定性对于生物催化和长期储存至关重要.
- 了解DES-酶相互作用是开发新保存策略的关键.
研究的目的:
- 评估DES作为酶储存的潜在媒介.
- 调查DES组成和含水量对酶稳定性的影响.
- 探索DESs中的酶的动态参数和结构变化.
主要方法:
- 综合和描述各种DESs.
- 在DESs中评估细胞酶和pectinase的稳定性.
- 分子模拟来分析酶动力学和结构动力学.
- 光谱法用于监测酶的二次结构变化.
主要成果:
- 在DES中酶的稳定性取决于含水量,呈现最佳范围.
- 增加的含水量最初增强,然后减少了酶的稳定性.
- DESs诱导了细胞酶和乳酸酶的二次结构变化.
- 分子模拟提供了对DESs.中的酶行为的洞察.
结论:
- 作为稳定剂,DES具有显著的潜力,可以长期保存酶.
- 这项研究为DES和酶的联合使用提供了理论框架.
- 优化DES中的水含量对于最大限度地提高酶稳定性至关重要.
相关概念视频
02:12Solvating Effects
An understanding of the solvating effect helps rationalize the relation between solvation and acidity of the compound. In addition, this also explains the relative stability of conjugate bases for compounds with different pKa values. This lesson details, in-depth, the principle of solvating effects. The strength of an acid and the stability of its corresponding conjugate base are determined using pKa values. This observed relationship is a consequence of solvation, which is the interaction...
01:15Cellulose and Pectic Polysaccharides
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...