菌体ISTP3的分离和表征,用于对抗耐药沙门氏菌的生物控制
Md Sharifull Islam1,2, Ishatur Nime1, Fan Pan2
1Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Frontiers in microbiology
|December 11, 2023
概括
一种新菌体,ISTP3,有效地减少了食品中的耐药沙门氏菌. 这种菌体表现出广泛的宿主范围和稳定性,在后抗生素时代为食品安全提供了有前途的生物控制剂.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 菌体研究 研究 菌体研究
背景情况:
- 沙门氏菌,包括耐药菌株,是重要的食物传播病原体.
- 在后抗生素时代,菌体正在成为生物抗菌剂.
研究的目的:
- 隔离和表征一种对抗耐药沙门氏菌有效的广泛菌体.
- 评估菌体ISTP3作为食品矩阵中的生物控制剂的疗效.
主要方法:
- 从家禽废水中分离和净化菌体.
- 使用传输电子显微镜和全基因组测序对菌体ISTP3的表征.
- 通过一阶段生长实验和稳定性测试来评估菌体稳定性和菌体活性.
- 在被耐药沙门氏菌污染的胸和生菜样本上评估菌体的疗效.
主要成果:
- 一种具有广泛宿主谱的菌体ISTP3 (阿克曼病毒家族) 被分离出来.
- 在各种pH值和温度条件下,ISTP3表现出高稳定性.
- 在12小时内在100或1000的MOI下,在4°C和25°C的胸和生菜上观察到耐药沙门氏菌数量的显著减少.
- 在ISTP3基因组中,缺乏溶解性,毒素或毒性基因.
结论:
- 菌体ISTP3是一种有前途的生物控制剂,用于预防和控制食品中的耐药沙门氏菌.
- 其广泛的宿主范围,性活性和稳定性使其适合食品安全应用.
- 在食品加工和生产中,ISTP3为抗生素提供了一种可行的替代方案,用于控制沙门氏菌.
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