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Updated: Jul 8, 2025

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
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生成一个新的sgRNA向量,pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR,以改善基编辑

Tolulope E Ayo1, Hao Xu1

  • 1Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, Mississippi 39406, United States of America.

Journal of genome editing and regulation
|December 11, 2023
PubMed
概括

研究人员开发了一个新的CRISPR/Cas9基编辑工具. 这种增强的sgRNA表达向量包括光和抗生素耐药性标记物,以便在基因编辑实验中更好地监测和选择细胞.

关键词:
在CRSPR/Cas9中使用.在Munc13-1-1中使用.在RBL-2H3中,使用RBL-2H3基础编辑 基础编辑mRFPFP是最多的一种.纯甲酸是一种纯甲酸.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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