在BCG上DosR的多方面的作用通过多omics透露了BCG
Yingying Cui1, Guanghui Dang1, Hui Wang1
1State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Frontiers in cellular and infection microbiology
|December 11, 2023
概括
休眠生存调节剂DosR显著影响Mycobacterium bovis BCG中的基因和蛋白质表达. DosR 删除改变了氨基酸合成,表明它是.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 结核病 (TB) 是一种由Mycobacterium tuberculosis (Mtb) 引起的传染性疾病.
- Mtb可以在宿主体内进入休眠状态.
- DosR (休眠生存调节器) 是一个关键的转录调节器,参与Mtb休眠和代谢.
研究的目的:
- 调查DosR在Mycobacterium bovis中扮演的角色和机制.
- 了解M. bovis BCG中DosR缺失的分子和代谢效应.
主要方法:
- 组合的奥米克分析 (转录和蛋白质组学).
- 针对氨基酸分析的向代谢组.
- 基因删除研究 (DosR淘汰赛).
主要成果:
- DosR 缺失影响了 104 个基因和 179 个蛋白质.
- DosR淘汰赛显著改变了氨基酸合成,上调L-酸和血清.
- 其他七种氨基酸,包括L-histidine和lysine,被降低调节.
结论:
- 在M. bovis BCG.中调节基因表达和蛋白质水平方面,DosR起着至关重要的作用.
- DosR 影响氨基酸代谢和合成途径.
- DosR代表了治疗干预的潜在新型监管目标.
更多相关视频
相关概念视频
02:02Genomics
36.4K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.4K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K