使用MALDI-TOF质谱仪快速检测耐甲林耐药黄金葡萄球菌中的抗菌素耐药性
Josiah J Rensner1, Paul Lueth2, Bryan H Bellaire2
1Department of Chemistry, Iowa State University, Ames, IA, United States.
Frontiers in cellular and infection microbiology
|December 11, 2023
概括
使用标签和MALDI质谱的快速抗菌感应性测试 (AST) 现在可以在短短3小时内检测Staphylococcus aureus中的甲素耐药性. 这一突破为传统方法提供了更快的替代方案,改善了患者的护理.
科学领域:
- 临床微生物学 临床微生物学
- 生物化学 生物化学
- 分析化学是一种分析化学.
背景情况:
- 抗菌素耐药性 (AMR) 对全球医疗保健构成重大威胁.
- 传统的抗微生物敏感性测试 (AST) 方法需要长时间的潜伏期,延迟关键的治疗决策.
- 需要快速的AST方法来打击抗药性病原体的增加.
研究的目的:
- 适应和验证一种使用标签和MALDI质谱检测的快速AST方法,用于检测耐美西林金黄色葡萄球菌 (MRSA).
- 评估使用低成本MALDI-TOF仪器快速检测MRSA的可行性.
- 与传统方法相比,大大减少AST的周转时间.
主要方法:
- 细菌在富含二氧化 (D2O) 的介质中培养.
- 用MALDI质谱测量融入新合成的脂质,检测出表明生长的质量转移.
- 该方法应用于对甲基西林敏感和对甲基西林耐药的金黄色细菌菌株,分析甲基西林存在时的质量转移.
主要成果:
- 经过3小时的甲基西林化后,在敏感的金黄色菌株中观察到平均脂质量的显著减少.
- 耐药S. aureus菌株表现出一致的乳标签,无论甲素度如何,表明没有抑制生长.
- 该试验成功地在3小时的时间内区分了敏感和耐药菌株.
结论:
- 乳标签和MALDI-TOF MS方法提供了快速而准确的AST检测,用于检测金黄色菌的甲素耐药性.
- 与传统的AST方法相比,这种方法可以大幅缩短交付时间.
- 由于其低成本和最小的样本处理要求,在临床实验室中广泛采用的潜力很高.
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