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Updated: Jul 8, 2025
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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
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在四种Leishmania物种中解码转录组复杂性的Iso-Seq转录的数据集.
Sandra González-de la Fuente1, Jose M Requena2,3, Begoña Aguado1
1Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Genomic and NGS Facility (GENGS), 28049 Madrid, Spain.
Data in brief
|December 11, 2023
概括
Iso-Seq技术揭示了四种Leishmania物种的全长转录,为基因调节提供了洞察力. 这些数据有助于开发利什曼病的新疗法,并指导有关寄生虫的分析.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 莱什曼尼亚寄生虫导致莱什曼尼亚病,这是一个广泛传播的疾病.
- 在Leishmania中,基因表达调节主要发生在转录后的水平上.
- 了解Leishmania转录组对于药物开发至关重要.
研究的目的:
- 用Iso-Seq.来描述四种Leishmania物种的全长转录组.
- 识别完整和截断的副本,包括那些有Spliced-Leader (SL) 序列的副本.
- 提供生物信息学管道,用于分析试类动物中的Iso-Seq数据.
主要方法:
- 总RNA从四种Leishmania物种的前列腺菌中提取出来.
- 单分子实时 (SMRT) 测序 (PacBio Iso-Seq) 用于转录测序.
- 转录的分类是基于SL序列和多A尾的存在.
主要成果:
- 高质量的,全长的成绩单产生了莱什马尼亚巴西人,L. donovani,L.婴儿,和L.主要.
- 确定了完整的转录 (含SL序列) 和截断的转录.
- 该研究为发现新型转录和替代拼接事件提供了有价值的数据.
结论:
- 生成的数据集增强了对Leishmania基因表达调节的理解.
- 这项研究可能有助于开发针对莱什曼病的新型治疗策略.
- 描述的生物信息学方法可以应用于其他试虫,如Trypanosoma cruzi和Trypanosoma brucei.
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