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Updated: Jul 8, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

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关于cryoEM数据存档和验证的社区建议:关于cryoEM数据管理,存储和验证的wwwPDB/EMDB研讨会的成果

Gerard J Kleywegt1, Paul D Adams2, Sarah J Butcher3

  • 1EMBL-EBI, Cambridge, UK.

ArXiv
|December 11, 2023
PubMed
概括

专家们聚集在一起,为冷电子显微镜 (cryoEM) 结构定义数据标准,重点是单粒子分析. 提出了改善结构生物学数据存储,验证和存档的建议.

关键词:
化样本电子显微镜 电子显微镜电子显微镜数据库电子显微镜数据库蛋白质数据库 蛋白质数据库数据存档数据的存档.质量控制质量控制质量控制验证验证的时间

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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