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用部分组装的TFIID复合体进行RNA聚合酶II转录

Vincent Hisler^1,2,3,4, Paul Bardot^1,2,3,4, Dylane Detilleux^1,2,3,4

¹Université de Strasbourg, IGBMC UMR 7104- UMR-S 1258, F-67400 Illkirch, France.

bioRxiv : the preprint server for biology

|December 11, 2023

概括

缺少TAF7或TAF10的部分组装的TFIID复合体可以启动RNA聚合酶II转录. 然而,这些TFIID变体在开发过程中并不能完全取代Holo-TFIID.

科学领域:

分子生物学分子生物学
基因法规基因法规
发育生物学发展生物学

背景情况:

RNA聚合酶II (Pol II) 转录启动对于基因表达至关重要.
一般的转录因子TFIID识别了核心促进子序列.
甲动物全体TFIID包括TATA结合蛋白 (TBP) 和13个TBP相关因子 (TAF).

研究的目的:

研究TAF7和TAF10在TFIID组装和功能中的作用.
确定TAF7或TAF10耗尽对转录启动和细胞表型的影响.
了解TFIID内部TAF的功能冗余性和必要性.

主要方法:

在小鼠胚胎干细胞和胚胎中对TAF7和TAF10进行诱导的淘汰模型.
分析TFIID复合组合和TAF占用率在发起人.
评估新生的Pol II转录和与染色体结合的TBP水平.

主要成果:

TAF7或TAF10的耗尽导致部分组装的TFIID复合体.
TAF 枯竭与改变的 TAF 占用率和明显的表型严重程度相关.
尽管TAF丢失,TBP仍然与染色质相关,新生的Pol II转录最初基本上不受影响.

结论:

部分组装的TFIID复合体能够维持基底Pol II转录启动.
完整的全体TFIID功能对于长期的细胞活力和发育至关重要.
在最初的转录步骤之外,TAF7和TAF10对于TFIID的完整性和完整功能至关重要.

关键词:

的RNA聚合酶II.在TAF10中,TAF10是TAF10.在TAF7中,TAF7是TAF7.这是一种TATA结合蛋白 (TBP).与TBP相关的因素 (TAFs)五分之一五分之一组装的组装组装的组装.胚胎干细胞 (ESC) 是一种质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量这里是鼠标鼠标鼠标鼠标鼠标鼠标.新生转录新生转录

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