Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Phosphatidylinositol diphosphate binding by ESCRT-III filaments.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets.

bioRxiv : the preprint server for biology·2026
Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026
Same author

Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titers.

Molecular therapy. Advances·2026
Same author

Indoor pollution induced oxinflammatory responses in lung tissues.

Chemico-biological interactions·2026
Same author

Improved sensors for fructose-1,6-bisphosphate enable in vivo imaging of glycolysis.

bioRxiv : the preprint server for biology·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jul 8, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

9.1K

一个化补充工具包用于询问脂质滴-有机体相互作用.

Xiao Li1, Rico Gamuyao1, Ming-Lun Wu1

  • 1Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, United States.

bioRxiv : the preprint server for biology
|December 11, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了FABCON,这是一个新的工具,可以实时可视化脂质滴和有机体之间的细胞接触点. 这种方法揭示了对细胞能量和脂质调节至关重要的动态相互作用.

更多相关视频

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.9K
A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

14.0K

相关实验视频

Last Updated: Jul 8, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

9.1K
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.9K
A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

14.0K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 代谢调节 代谢调节 代谢调节

背景情况:

  • 器官接触点对于细胞脂质和能量平衡至关重要.
  • 随着时间的推移,在活细胞中可视化这些纳米级相互作用带来了重大的技术挑战.

研究的目的:

  • 开发一种用于检测和分析活细胞中器官接触点的新型工具包.
  • 在不同的代谢条件下研究脂质滴-有机体相互作用的动态性质.

主要方法:

  • 使用分FAST光蛋白开发FABCON (在接触点的素激活双分子补充) 的开发.
  • 细胞内膜网膜 (ER) -线粒体 (线粒体) -脂质滴接触点的定量分析.
  • 应用自动化管道来按尺寸分类接触点.

主要成果:

  • 通过FABCON,可以对有机体接触部位进行最小干扰可视化.
  • 在脂质代谢过程中,ER-mito-lipid滴滴接触的动态变化得到证明.
  • 接触部位的分类揭示了可能表明不同调节和功能的子组.

结论:

  • FABCON为脂质滴-有机体接触点的动态调节提供了新的见解.
  • 该工具可用于将各种有机体接触部位可视化,包括ER-mito.
  • 这些发现为进一步对代谢调节的机制研究提出了假设.