同时的CRISPR查和空间转录学揭示了细胞内,细胞间和功能转录电路
Loϊc Binan1, Serwah Danquah1,2, Vera Valakh1,2
1Spatial Technology Platform, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
bioRxiv : the preprint server for biology
|December 11, 2023
概括
Perturb-FISH将成像空间转录学与指导RNA检测相结合,以研究基因功能. 这种方法揭示了对先天免疫力和自闭症谱系障碍风险基因的新见解.
科学领域:
- 细胞生物学 细胞生物学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 聚合光学屏幕提供大规模的细胞分析,但缺乏转录细节.
- 高复合单细胞转录组学可以告知分子通路,但往往缺乏空间上下文.
结论:
- Perturb-FISH是一种用于剖析空间和功能生物学遗传和分子基础的多功能方法.
- 通过整合转录组,空间和功能数据,推进复杂生物系统的研究.
- 开辟了了解基因功能在发育,疾病和免疫系统中的新途径.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...