植物界甲基的兰化物依赖的隔离选择了植物遗传学上保存但代谢上多样化的社区
Alekhya M Govindaraju1, Colleen A Friel2, Nathan M Good1
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA.
bioRxiv : the preprint server for biology
|December 11, 2023
概括
兰他尼德生物化学影响植物叶中的甲基. 密切相关的细菌表现出多样化的代谢能力和对这些金属的多样化反应,这表明了植物界殖民的利基分割.
科学领域:
- 微生物生态学 微生物生态学
- 生物化学 生物化学
- 植物与微生物的相互作用
背景情况:
- 甲基型细菌对于植物植物界的碳循环至关重要.
- 兰化物可以影响微生物的新陈代谢,但它们在植物界甲基类生物中的特定作用尚未得到研究.
研究的目的:
- 为了研究兰化物生物化学如何影响甲基类植物圈社区的组成和代谢潜力.
- 探索大豆叶中的Methylobacterium菌株的代谢多样性和利基区分策略.
主要方法:
- 从大豆叶子中分离甲醇氧化细菌.
- 使用16srRNA和rpoB基因进行了家族遗传学分析.
- 评估各种碳基板上的生长情况,含有和不含有兰化物.
- 选择菌株的全基因组测序.
主要成果:
- 在344个分离物中没有发现有义务依赖兰他尼德的甲基类动物.
- 遗传学分析显示,在Methylobacterium extorquens clade中具有很高的相似性.
- 在具有相同酶的菌株中观察到显著的代谢分歧,包括在兰化物影响的酒精上产生差异性生长.
- 一些菌株缺乏已知的甲酸相关基因.
- 显著的百分比的分离物可以代谢糖和芳香酸.
结论:
- 即使在密切相关的Methylobacterium菌株中,代谢能力和兰化物利用率也存在显著差异.
- 这些差异表明,利基的分区策略使得植物界殖民成为可能.
- 这项研究扩大了Methylobacterium在植物圈中已知的代谢谱.
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