视觉空间转录学揭示了瘤内部异质性和前列腺癌格里森分数进展的概况
Yongjun Quan1, Hong Zhang2, Mingdong Wang1
1Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing 100176, China.
iScience
|December 11, 2023
概括
这项研究使用空间转录学来分析前列腺癌 (PCa) 组织. 它揭示了参与格里森分数进展的关键基因和生物途径,提供了对多焦PCa发展的见解.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 前列腺癌 (PCa) 通常在单个腺体内表现出多焦点性疾病.
- 了解驱动格里森评分 (GS) 进展的分子机制对于PCa管理至关重要.
研究的目的:
- 分析PCa组织中腺上皮细胞 (GE) 的全转录组概况,使用不同的格里森分数.
- 调查空间转录组模式,并识别与格里森分数升级相关的基因.
主要方法:
- 利用Visium空间转录学 (ST) 来分析四个PCa组织中的GE细胞.
- 在不同的GS中分析了遗传分类,空间模式和推断的副本数变异 (inferCNV).
- 在GS进展过程中评估了发育轨迹并确定了差异表达基因 (DEG).
主要成果:
- 遗传分类与不同GS的组织结构的空间模式相关.
- 平均推断CNV值随着GS的发展而增加.
- 确定了与GS升级相关的关键基因 (NANS,PABPC1L,PILRB,PPFIA2,SESN3) 并揭示了DEGs的患者特异性.
结论:
- 空间转录组学为前列腺癌 Gleason分数进展期间的转录组范围的表达模式提供了宝贵的见解.
- 确定了与PCa进展相关的特定基因和生物功能,包括卡德林结合和细胞粘附.
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Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
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