周围动脉斑块中的体会导致内皮细胞功能障碍
Rodrigo Meade1, Yang Chao1, Nikolai Harroun1
1Section of Vascular Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO.
JVS-vascular science
|December 11, 2023
概括
糖尿病患者外周动脉中胺C16的升高会损害内皮细胞功能,这表明它是外周动脉疾病严重程度的生物标志物.
科学领域:
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 血管生物学 血管生物学
- 糖尿病并发症 糖尿病并发症
背景情况:
- 周围动脉疾病 (PAD) 和其严重形式,慢性四肢威胁性缺血症 (CLTI) 很普遍,特别是在糖尿病患者中.
- 糖尿病患者晚期PAD的动脉组织中生物活性脂质发生变化.
研究的目的:
- 评估脂胺18:1/16:0 (C16) 作为糖尿病患者内皮细胞和外围动脉中细胞调节者的作用.
- 为了研究C16水平,动脉疾病严重程度和糖尿病中的内皮细胞功能之间的联系.
主要方法:
- 在患有和没有糖尿病的患者的带动脉组织中分析了胺C16水平.
- 评估基因化酶 (SMPD) 和胺合成酶 (CERS) 的基因表达.
- 使用暴露于C16的人类静脉内皮细胞 (HUVEC) 的体外研究,包括增殖,亡和自分析.
- 在HUVEC中选择性淘汰SMPD2和SMPD4.
主要成果:
- 胺C16是糖尿病患者的小腿动脉中胺含量最高的 (11%的增加).
- C16水平与基胺 (SPM) 含量呈正相关性.
- 在患病的小腿动脉中观察到SMPD表达的升高,特别是SMPD4.
- 外源C16降低了HUVEC的扩散,增加了亡,并降低了自.
- 在C16暴露后,SMPD4敲除挽救了细胞增殖.
结论:
- 胺C16作为一个组织生物标志物用于糖尿病中PAD严重程度.
- C16显著影响内皮细胞活力和功能,突出其在糖尿病血管并发症中的作用.
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