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在健康的儿科人口中Treg和Breg细胞的变化

Yiyi Luo^1,2,3, Daniel Acevedo^1,2,3, Alexandru Vlagea^2,4

¹Clinical Immunology and Primary Immunodeficiencies Unit, Allergy and Clinical Immunology Department, Hospital Sant Joan de Déu, Barcelona, Spain.

Frontiers in immunology

|December 11, 2023

概括

确定儿科调节性T细胞 (Treg) 和调节性B细胞 (Breg) 的参考值对于诊断免疫障碍至关重要. 这项研究定义了Treg表型,并分析了儿童的Treg和Breg随年龄的变化.

科学领域:

免疫学免疫学免疫学
儿科儿科儿科
流动细胞计量 (Flow Cytometry) 是一种流动细胞计.

背景情况:

临床诊断免疫的先天性错误,包括Tregopathies,需要儿童调节性T细胞 (Treg) 的年龄分层参考值.
缺乏标准化的Treg免疫表型定义,阻碍了对诊断结果的准确解释.
调节性B细胞 (Breg) 在儿科群体中未得到充分研究,尽管它们在免疫调节中的作用.

研究的目的:

分析Treg细胞不同免疫表型定义之间的相关性.
对健康儿童的Treg和Breg频率及其成熟状态的年龄相关变化进行调查.
建立Treg和Breg在儿科诊断中的年龄分层参考值的基础.

主要方法:

在55名健康儿科对照中,使用流细胞计进行了Treg和Breg的外周血液免疫类型鉴定.
分析了三种Treg免疫类型的定义 (CD4+CD25hiCD127low,CD4+CD25hiCD127lowFoxP3+,CD4+CD25hiFoxP3+) 和Breg (CD19+CD24hiCD38hi) 进行了分析.
在不同年龄组中评估了Treg和Breg的频率和成熟状态.

主要成果:

根据使用的免疫类型定义,Treg数量有很大的差异,频率从0.07%到9.7%不等.
大多数Treg定义,特别是细胞内面板之间观察到正相关性.
随着年龄的增长,Treg和Breg频率通常分别在7岁和3岁后随着年龄的增长而下降,而Treg成熟度则随着年龄的增长而增加.

结论:

对Treg频率的解释需要仔细考虑所使用的免疫表型定义,尽管定义之间的相关性很好.
在儿科对照中发现了Treg和Breg频率和成熟模式的与年龄相关的变化.
随着年龄的增长,调节性B细胞的动态似乎先于儿童的调节性T细胞.

关键词:

布雷格细胞是什么?福克斯P3P3 在线播放 IPEXEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX IPEX 在Treg细胞中.孩子孩子孩子孩子孩子孩子免疫表因型的确定

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