对过敏原表达B细胞的采用转移可以防止IgE介导的过敏
Lisa Prickler1, Ulrike Baranyi2, Konstantinos Mengrelis1
1Division of Transplantation, Department of General Surgery, Medical University of Vienna, Vienna, Austria.
Frontiers in immunology
|December 11, 2023
概括
转移过敏原表达B细胞可以防止草花粉过敏的发展. 这种细胞疗法方法对预防人类IgE介导过敏有前途.
科学领域:
- 免疫学 免疫学 免疫学
- 过敏研究 研究过敏
- 细胞疗法细胞疗法
背景情况:
- 建立耐受性以预防过敏是一个重要的未满足的医疗需求.
- 之前的研究表明,造血干细胞转移诱导过敏原特异性耐受性.
- 调查其他免疫细胞对过敏原特异性耐受性诱导至关重要.
研究的目的:
- 为了评估过敏原表达免疫细胞诱导过敏原特异性耐受性.
- 为了确定CD19+B细胞作为潜在的候选人对过敏原特异性细胞疗法.
- 在草花粉过敏的小鼠模型中评估CD19+B细胞转移的疗效.
主要方法:
- 通过将Phlp5表达的CD19+B细胞转移到原始小鼠体内.
- 小鼠接受了拉帕素和抗CD40L抗体的预治疗.
- 进行过敏原敏感化,随后对免疫反应和肺炎进行分析.
主要成果:
- 转移Phlp5表达CD19+B细胞诱导了B细胞奇默症长达三个月.
- 预防了Phlp 5特定IgE和IgG1抗体反应的发展,持续了26周.
- 接受治疗的小鼠显示肺功能得到保护,并且没有巨细胞脱粒,这表明特定的耐受性.
结论:
- 在小鼠模型中,Phlp5表达的CD19+B细胞的转移会诱导过敏原特异性耐受性.
- 这种方法有可能用于预防方案,以预防人类的IgE介导过敏.
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