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巨细胞IL-1β阳性微囊表现出血栓炎性质,可检测到患有活跃青少年异常性关节炎的患者

Audrey Cambon^1,2, Charlotte Rebelle^1,3, Richard Bachelier¹

¹Aix-Marseille University, Institut National de la Santé Et de la Recherche Médicale (INSERM), Institut National de la Recherche pour l'Agriculture et l'Environnement (INRAE), Centre de Recherche en CardioVasculaire et Nutrition (C2VN), Marseille, France.

Frontiers in immunology

|December 11, 2023

概括

携带互白素-1β (IL-1β) 的白细胞微囊 (MVs) 是被激活的巨细胞分泌的. 这些IL-1β阳性MV,含有炎症组分和组织因子,可在青少年异常性关节炎患者中检测到.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

介乐金-1β (IL-1β) 是一种关键的炎症性细胞因子,分泌途径尚不清楚.
在患者血清中,IL-1β通常是不可检测的,即使在IL-1β介导的疾病中,如系统性青少年异常性关节炎 (sJIA).
白细胞微囊 (MVs) 被认为是IL-1β分泌的潜在机制.

研究的目的:

从巨细胞培养物中鉴定IL-1β阳性MV的特征,并评估它们的生物功能.
在患有青少年异常性关节炎 (JIA) 的患者中调查循环IL-1β阳性MV的存在.

主要方法:

从THP-1衍生的巨细胞中净化MVs,使用LPS±ATP刺激.
通过ELISA,西部斑点和流细胞计量对IL-1β,NLRP3炎症体,卡斯巴酶-1,P2X7受体和组织因子 (TF) 的MV含量分析.
在体外评估MV的促炎性质和在体内评估白细胞招募在小鼠腹膜炎模型.

主要成果:

激活的巨细胞释放含有IL-1β (前体和成熟形式),NLRP3,caspase-1和生物活性TF的MV.
具有IL-1β阳性的MVs表达了P2X7受体,并在ATP刺激后释放可溶性IL-1β.
在体内,MVs诱导白细胞透,在活跃的JIA患者的血中检测到IL-1β阳性MVs.

结论:

被激活的巨细胞释放的微囊作为血栓炎症载体.
这些含有IL-1β的MVs携带炎症组分和组织因子.
循环IL-1β阳性MV可在患有活跃青少年异常性关节炎的患者中检测到.

关键词:

一个IL-1ββ.在NLRP3炎症基因组中,NLRP3是NLRP3-炎症基因.青少年异常性关节炎 (Idiopathic Arthritis) 是一种疾病.微小的微小的微小的微小的微小.组织因子是组织因子.

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