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基于miRNA-基因整合分析的结肠癌关键miRNAs的识别和验证

Yi Ji¹, Jialin Gu^1,2, Hongqun Zhang^1,2

¹Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, People's Republic of China.

International journal of general medicine

|December 11, 2023

概括

这项研究确定了三种关键的微RNA (miRNA) 参与结肠癌的发展. 这些发现可能有助于早期诊断和针对结肠癌的向治疗.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

微RNAs (miRNAs) 在结肠癌的发展中至关重要.
识别特定的miRNA可以帮助早期诊断和治疗策略.

研究的目的:

确定和验证关键的微RNA (miRNA) 作为结肠癌的潜在生物标志物.
探索这些miRNAs,免疫细胞和免疫治疗之间的关系.

主要方法:

从基因表达综合 (GEO) 数据库中分析了两个miRNA微阵列数据集 (GSE53339,GSE126093).
利用生物信息学工具 (limma,miRwalk,Cytoscape) 进行差异表达分析,目标预测和网络构建.
使用定量实时PCR (qRT-PCR) 和癌症基因组图谱 (TCGA) 数据验证的关键miRNA.

主要成果:

确定了14个常见的微分表达miRNAs (DEMis).
构建了一个miRNA基因网络,确定了三个关键miRNA:miR-363-3p,miR-520e和miR-330-5p.
miR-520e与透免疫细胞和免疫检查点有显著的关联.

结论:

三个关键的miRNAs (miR-363-3p,miR-520e,miR-330-5p) 被确定为影响结肠癌的发病因子.
透的免疫细胞似乎在结肠癌中发挥着重要作用.
这些发现支持早期诊断和精确向治疗结肠癌.

关键词:

地理地质组织 (GEO) 是指地理地质组织.结肠癌是什么意思结肠癌是什么意思免疫细胞的透.这是一个小RNARNA.qRT-PCR 是一个很好的方法.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...