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功能识别miR159a,一个积极的调节器在树对干旱压力抵抗期间

Tiantian Fu¹, Chun Wang¹, Yuzhang Yang¹

¹State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.

Horticulture research

|December 11, 2023

概括

微RNA 159a (miR159a) 通过减少口腔开口和提高用水效率来提高树的干旱耐受性. 过度表达miR159a可以改善干旱条件下的植物生存,为树木繁殖提供潜力.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

干旱压力对植物生长和发育产生重大影响.
微RNAs (miRNAs) 是植物应激反应的关键调节者,但它们在木质植物中的作用仍未得到充分探索.
MiR159是一种保存的miRNA家族,涉及植物生长和应激适应.

研究的目的:

为了研究miR159a在树 (Populus spp.) 中的功能. 在干旱压力下.
为了确定干旱期间受miR159a影响的基因和调节途径.
评估miR159a在改善树木耐旱能力方面的潜力.

主要方法:

干旱处理适用于野生型和转基因木种 (过度表达miR159a和miR159a抑制).
生理测量包括口腔孔径,用水效率和反应性氧物种清理等.
分子分析包括RT-qPCR,降解组测序,5'RACE和转录组学.

主要成果:

miR159a的表达因树的干旱而上调.
过度表达miR159a减少了口腔开口,提高了用水效率,并在干旱期间改善了反应性氧物种的清理.
确定MYB基因是miR159a的直接目标,miR159a抑制增加了干旱敏感性.

结论:

miR159a在赋予树干旱耐受性方面发挥着至关重要的作用.
它通过调节口腔关闭,树发育和用水效率来发挥作用.
miR159a及其向基因代表了繁殖耐旱树木的有价值的向.

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