circ-ATAD1作为对 miR-191-5p 的竞争性内源RNA 力量 非小细胞肺癌的进展
Zhihua Wan1, Shanshan Jia2, Junhua Lu2
1Baoding First Central Hospital, Baoding City, 071000, Hebei Province, China.
Applied biochemistry and biotechnology
|December 11, 2023
概括
循环RNAcirc-ATAD1通过海绵化miR-191-5p促进非小细胞肺癌 (NSCLC) 的进展,导致叉头盒K1 (FOXK1) 表达的增加. 这种机制突出了circ-ATAD1作为NSCLC的潜在治疗点.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在各种癌症中的作用,包括非小细胞肺癌 (NSCLC).
- 环RNAs影响NSCLC进展的特定机制需要进一步阐明.
研究的目的:
- 研究circ-ATAD1在非小细胞肺癌 (NSCLC) 中的作用和机制.
- 探索circ-ATAD1表达和NSCLC患者预后之间的关系.
- 阐明涉及NSCLC细胞中circ-ATAD1,miR-191-5p和FOXK1的调节途径.
主要方法:
- 在NSCLC组织中对circ-ATAD1的临床表达分析和与预后的相关性.
- 在体外实验中使用A549细胞与circ-ATAD1和miR-191-5p操纵.
- 对细胞增殖 (CCK-8,殖民地形成),细胞亡 (流细胞计),迁移 (伤口愈合) 和入侵 (Transwell) 的测试;使用双露西法酶记者测试和星基分析验证分子相互作用.
主要成果:
- 在NSCLC组织中,Circ-ATAD1显著上调,并与预后不佳有关.
- Circ-ATAD1的过度表达促进了NSCLC细胞的增殖,迁移和入侵,而它的下调抑制了这些过程.
- Circ-ATAD1作为miR-191-5p的海绵,可以负面调节FOXK1.1. 对FOXK1的上调或对miR-191-5p的下调挽救了circ-ATAD1下调的影响.
结论:
- 通过隔离miR-191-5p,Circ-ATAD1加速NSCLC的进展,从而上调Forkhead Box K1 (FOXK1) 的表达.
- Circ-ATAD1作为NSCLC预后和治疗点的潜在生物标志物.
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