相关实验视频

Updated: Jul 8, 2025

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

PLIN1和MOGAT1基因的多态性及其与胡羊的料效率的关联

Zongwu Ma¹, Weimin Wang², Deyin Zhang²

¹College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.

|December 11, 2023

概括

研究人员在胡羊中发现了两种基因突变,这些突变显著影响了料效率. 这些遗传标记物PLIN1 (g.18517910 A>G) 和MOGAT1 (g.224856118 G>C) 可以帮助繁殖计划改善料利用率并降低生产成本.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

料成本占绵羊生产成本的很大一部分.
提高羊的料利用率对于降低成本和提高经济可行性至关重要.
识别用于料效率的遗传标记可以促进胡羊的选择性繁殖.

研究的目的:

为了研究各种胡羊组织中PLIN1和MOGAT1基因的表达模式.
探索PLIN1和MOGAT1基因中的多态性和胡羊的料效率特征之间的关联.
确定潜在的分子遗传标记,以提高胡羊养殖计划中的料利用率.

主要方法:

使用定量实时PCR (qRT-PCR) 来确定多种组织的基因表达水平.
通过PCR放大和桑格测序,在PLIN1和MOGAT1基因中发现了特定突变.
马萨雷 (MassARRAY®) SNP检测技术对985只胡羊进行基因定型,以分析SNP与料效率参数 (BW80,BW180,FI,ADFI,ADG,FCR) 之间的关联.

主要成果:

PLIN1和MOGAT1基因表现出广泛的组织表达,PLIN1在尾巴脂肪中表达最高,MOGAT1特别表达在肝脏,尾巴脂肪,肺和心脏中.
发现了两个内基突变,即PLIN1中的g.18517910 A>G和MOGAT1中的g.224856118 G>C.
SNP g.18517910 A>G与体重,料摄入量和料转化率 (P<0.05) 有意义地相关.
SNP g.224856118 G>C与料转化率 (P<0.05) 有意义地相关.
基因型组合也显示出显著差异 (P<0.05),表明复杂的遗传影响.

结论:

已确定的SNP,PLIN1中的g.18517910 A>G和MOGAT1中的g.224856118 G>C,是胡羊料效率特征的重要分子标记.
这些遗传标记物有可能用于标记物辅助选择,以提高胡羊养殖中料利用效率.
进一步的研究可以探索这些关联背后的功能机制,以优化育种策略.

关键词:

料效率料效率是指料的使用效率.胡羊胡羊胡羊是什么意思 MOGAT1 的意思是在 PLIN1 里.单个核酸的多态性.

更多相关视频

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Published on: February 14, 2016

相关概念视频

Incomplete Dominance

Incomplete Dominance

Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.

Epistasis

Epistasis

In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...