尼与homocysteine thiolactone的修改及其对抗微生物活性的影响
Ezgi Demir Özer1, Metin Yildirim2
1Department of Gastronomy and Culinary Arts, Cappadocia University, Nevşehir, Turkey. ezgi.ozer@kapadokya.edu.tr.
概括
尼辛被修改了同类氨酸 thiolactone,以创建一个N-同类氨酸结合物. 这种修改后的尼显示出对大多数测试细菌的抗菌活性降低,除了Enterococcus faecium.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
背景情况:
- 尼辛是一种具有广谱抗菌活性的细菌素.
- 尼的化学修饰可以改变其特性和活性.
- N-homocysteinylation是修改nisin的一种潜在策略.
研究的目的:
- 为了准备尼的N-homocysteine结合物.
- 评估N-homocysteinylation对尼抗菌活性的影响.
- 为了确定尼修饰的最佳条件.
主要方法:
- 在各种条件下,Nisin与同类氨酸 thiolactone 结合.
- 使用乙酸尿素聚烯胺凝电泳 (AAU-PAGE) 和三二硫酸二聚烯胺凝电泳 (三SDS-PAGE) 监测了修饰.
- 抗菌活性测试针对黄金葡萄球菌,菌菌,细菌,乳球菌,Listeria monocytogenes和大肠杆菌.
主要成果:
- 确定了最佳的N-homocysteinylation条件 (6毫克/毫升尼辛,150毫克的homocysteine thiolactone,pH为3.0,6小时).
- 修改后的尼对大多数菌株的抗菌活性显著降低,除了Enterococcus faecium.
- 修改后的尼抑制Enterococcus faecium的抑制率大约是未修改后的尼的10%.
- 在酶性水解和同型半氨酸-thiolactone反应后,在Lys12和Lys22发生了特定的侧链修饰.
结论:
- 尼的N-homocysteinylation并没有增强其整体抗菌活性.
- 这种修改主要影响了Enterococcus faecium,降低了它的易感性.
- 对特定修改及其对活动的影响进行进一步调查是有必要的.
相关概念视频
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Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...
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